Development of a Gold Nanoparticle Based Lateral Flow Assay for Rapid Diagnosis of Contagious Agalactia in Goats

Contagious agalactia of sheep and goats

Diagnosis can be performed in any laboratory which has the appropriate culture media. Identification of the three mycoplasmas responsible is relatively easy on the basis of cultural and biochemical characteristics, confirmed serologically. A clinical diagnosis within a herd can be confirmed by detecting serum antibodies by the complement fixation or ELISA techniques, which are also suitable for...

ENHANCED PERFORMANCE OF A LATERAL FLOW ASSAY Use of a novel conjugate blocking technology to improve performance of a gold nanoparticle-based lateral flow assay

A Gold Nanoparticle and Aflatoxin B1-BSA Conjugates Based Lateral Flow Assay Method for the Analysis of Aflatoxin B1

A rapid and simple immuno-chromatographic assay was developed to detect aflatoxin B1 (AFB1). The assay was based on a modified competitive binding format using colloidal gold and polyclonal antibody (Pab) conjugates. The anti-AFB1 Pab was immobilized to a defined detection zone on a porous nitrocellulose membrane and colloidal gold particles were conjugated to AFB1-BSA which served as a detecti...

A dual gold nanoparticle conjugate-based lateral flow assay (LFA) method for the analysis of troponin I.

For signal amplification without an additional operation step in a gold nanoparticle (AuNP)-based lateral flow assay (LFA), a new and simple method utilizing two AuNP-antibody conjugates was developed. The 1st conjugate was the AuNP immobilized with an anti-troponin I antibody and blocked with bovine serum albumin (BSA), and the 2nd conjugate was the AuNP immobilized with an anti-BSA antibody a...

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اشعار ریچ، به عنوان اشعاری که همیشه در حال تغییر و دگرگونی هستند، تجسمی از رشد و دگرگونیِ انسان هستد. پایان نامه ی حاضر، با تمرکز بر روی مراحل سیر شعری ریچ از تغییری در دنیا به عکس هایی فوری از یک عروس، سپس به شیرجه به درون کشتی شکسته و در نهایت به صبری عجیب مرا تا اینجا آورده، به بررسی این مراحل در قالب نظر شوالتر در رابطه با سه مرحله ی پیشرفت ادبی زنان یعنی مرحله ی زنانه، زن گرا و زن محور می پ...